FastQCFastQC Report
Thu 26 May 2016
ERR1357010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251871
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA5050.028.00471966
GTATCAA58300.027.6043241
CTCGATA5050.026.63863665
CGATAAT5350.025.78962167
TCGTCTA5150.025.45442654
GCTCGAT5550.024.23929464
GGTATCA35950.023.7278211
CGTCTAC6100.023.1868155
CGTCATT6550.023.1697426
ATCAACG69750.022.254443
CAACGCA71550.021.742795
TGGTCGT6350.021.73550251
TATCAAC72550.021.6808262
GCGTAAT800.002878426821.59063311
CATTGCG7200.021.55707629
GCTCGTC6900.021.49458939
TCAACGC74550.020.960374
AACGCAG76100.020.7442426
GTCGTCT6550.020.53681653
TCGGTAT5050.020.48989327
Produced by FastQC (version 0.11.5)