FastQCFastQC Report
Thu 26 May 2016
ERR1357007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324061
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG4000.032.77044319
CGTTAAC1800.032.6095961
ACATACG652.3239949E-531.84172417
CGACGAG4250.030.84276824
ATCGTCA4250.030.84276810
TGAACCG756.143113E-527.6138945
TGGTCGT6500.027.60763451
GCTCGTC6650.027.49137739
GTCAGCG6750.027.0881943
GTCTACC7150.026.54479856
GCGCTGG7050.026.42585247
GTCGTCT6850.026.19801553
CTGGTCG7150.026.06315250
GCTCGAT7100.025.7528664
CGGGATA6050.025.66825350
GTACTAG6000.025.3203931
GTCTAGG1101.144932E-625.1111341
CTCGATA7300.025.04730265
GCGCGAT3450.024.99652513
TCGATAA7400.024.70882666
Produced by FastQC (version 0.11.5)