FastQCFastQC Report
Thu 26 May 2016
ERR1357007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324061
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4200.036.13138624
TGGTCGT6300.033.41687451
ATCGTCA4650.032.68911410
CAATGCG4700.032.28761719
GTACTAA751.6476515E-632.2543371
GTCAGCG6800.030.43151343
CTGGTCG7100.030.13768650
TTCGGTC1401.4551915E-1129.58215149
GCTCGTC7100.029.14567439
GTCGTCT7050.028.86317453
GTCTACC7100.028.6664156
ATCGTGG5800.027.9480234
GTCGGTC500.007684712327.63715712
GCGCTGG7750.027.61000847
TCGGTCC1503.45608E-1127.61000650
TCGTCTA7400.027.5042654
GCTCGAT7450.027.31966664
CTCGATA7600.027.23128165
CGTGGTT6000.027.01642236
CGTCATT7550.026.9517126
Produced by FastQC (version 0.11.5)