ERR1357005_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1357005_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448880
Sequences flagged as poor quality	0
Sequence length	75
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	2819	0.6280074852967386	RNA PCR Primer, Index 31 (96% over 29bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG	963	0.21453395116734983	No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA	710	0.15817144893958296	RNA PCR Primer, Index 31 (96% over 29bp)
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT	698	0.15549812867581536	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT	659	0.14680983781857065	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA	658	0.14658706112992337	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG	647	0.14413651755480306	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	628	0.13990376047050437	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT	625	0.13923543040456246	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG	577	0.12854214934949207	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	563	0.12542327570842987	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC	548	0.12208162537872037	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC	530	0.11807164498306896	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	497	0.11072001425770808	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT	490	0.10916057743717697	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAAT	480	0.10693281055070397	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCA	472	0.10515059704152559	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA	464	0.10336838353234717	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA	458	0.10203172340046338	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATCA	15	0.0023368658	69.13172	1
GATGGGC	15	0.0023565849	68.985405	38
ACGGGAT	30	9.1284255E-6	57.48784	67
CCACGGG	30	9.1284255E-6	57.48784	65
CCTTTAG	25	2.5681767E-4	55.188324	25
CTCACGC	20	0.007355798	51.750584	56
AATCCAT	20	0.0073622824	51.739056	37
GGTATTC	20	0.0073622824	51.739056	13
TGAGGGC	20	0.0073622824	51.739056	34
GTACCCA	30	6.2483083E-4	46.087814	1
ATGTTGC	40	4.967294E-5	43.168774	8
AGTCCAC	40	5.0000846E-5	43.12068	62
GTCCACG	40	5.0000846E-5	43.12068	63
TCCACGG	40	5.0033734E-5	43.11588	64
GCTAGGC	40	5.0033734E-5	43.11588	32
GGCCTTA	40	5.0033734E-5	43.11588	36
GAAGGCG	35	0.0013483426	39.42023	31
TAGCAGA	45	1.0005239E-4	38.333767	46
TATGTTA	45	1.0011807E-4	38.329494	5
GACAGGT	55	7.395065E-6	37.628403	19

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content