FastQCFastQC Report
Thu 26 May 2016
ERR1357004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134541
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG3950.040.1730324
CAATGCG4250.038.14896819
CTCGATA6100.036.1945565
GCTCGAT6200.036.16718364
ATCGTCA4700.035.9643410
CGTCATT6500.035.55626726
GCTCGTC6450.035.29709639
GTCTACC6450.034.77608556
GTCAGCG6550.034.7612743
TGGTCGT6550.034.2451551
GTCGTCT6700.033.99352353
TCGATAA6500.033.9671966
TCGTCTA6800.033.49361854
TCAGCGC6800.033.48328444
CGATAAT6950.033.25699667
CGTCTAC6850.033.24913855
CATTGCG7250.032.82961729
CTGGTCG6900.032.5080850
AGTCCTA4900.031.67897829
AGGTCGT7350.031.44570422
Produced by FastQC (version 0.11.5)