FastQCFastQC Report
Thu 26 May 2016
ERR1357004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134541
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG3850.043.8982424
CAATGCG4200.041.0612819
CTGGTCG7250.038.0729850
TGGTCGT7250.037.59706551
GCTCGAT7300.037.32803364
TCAGCGC7500.036.790944
CTCGATA7450.036.11187465
GCTCGTC7650.036.0695139
GTCAGCG7750.036.04915243
ATCGTCA4750.035.63564710
CGTCATT7900.035.36467426
TGCGACG4900.035.19537722
CGATAAT7750.034.71399767
GCGCTGG8350.034.2970347
TCGTCTA8050.034.28026254
GTCTACC8100.034.06865356
CATTGCG8350.033.87186429
TCGATAA8000.033.62918566
AGGTCGT8400.033.25963622
GTCGTCT8250.033.028253
Produced by FastQC (version 0.11.5)