FastQCFastQC Report
Thu 26 May 2016
ERR1356998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325216
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA5850.034.20314465
GCTCGAT5900.033.91328464
TGGTCGT6100.032.80756851
TCGATAA6100.032.23583666
CTGGTCG6350.031.51593250
CAATGCG3900.030.95857419
GTCAGCG6500.030.78283143
GTCGTCT6550.030.5559253
TCGTCTA6700.030.38686454
GCTCGTC6550.030.01662639
CGATAAT6600.029.27102967
CGCGTAA605.424481E-428.74833165
TCAGCGC7000.028.58405944
CGACGAG4000.028.46084824
ATCGTCA4300.028.07870510
CATTGCG7400.027.5020529
GCGCTGG7350.027.22393847
CGTCATT7700.025.98452626
TATTCGC809.569136E-525.8725229
GTCTACC7750.025.8246856
Produced by FastQC (version 0.11.5)