FastQCFastQC Report
Thu 26 May 2016
ERR1356998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325216
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATACG404.9960792E-543.134349
CGACGAG4600.035.98738524
CCGTCGT400.002575537234.5570149
ATCGTCA4800.034.5570110
CGCAATC709.755822E-734.491813
TGGTCGT7100.034.02141651
CTCGATA7000.034.00035565
CTGGTCG7300.033.5620350
GTCAGCG7500.033.10838743
GCTCGAT7300.032.6043164
TCAGCGC7750.032.48538244
CAATGCG5250.031.53180119
TCGTCTA7800.031.40113354
TCTTACG553.261831E-431.3561942
TCGATAA7650.031.11143566
GTCGTCT7950.030.80052253
GCGCTGG8350.030.58144447
CGATAAT7900.029.69027567
GTCTACC8400.029.15929656
TGCGACG5800.029.13633522
Produced by FastQC (version 0.11.5)