FastQCFastQC Report
Thu 26 May 2016
ERR1356996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502976
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATT6850.033.2351126
CGACGAG4900.033.08940524
CTCGATA6800.031.45419765
CGATAAT6700.031.40876667
TGGTCGT6850.030.22400351
TCGATAA7700.028.2257666
TGCGACG5750.028.19792622
GCTCGTC7950.027.76821339
CTGGTCG7850.027.69250150
GCTCGAT7600.027.68930664
CAATGCG6100.027.14500219
GTCGTCT7450.026.86567553
GTCAGCG8800.026.26616343
TCGTCTA7800.026.1025854
AGGTCGT8350.026.02802722
ATGCGAC6500.026.00577721
CATTGCG8250.025.9222629
GTCGTCA8600.025.6725324
ATCGTCA6950.024.81782310
GTACTAG8650.024.7497461
Produced by FastQC (version 0.11.5)