FastQCFastQC Report
Thu 26 May 2016
ERR1356996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502976
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA51050.20395720933800407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT7600.033.12882267
TGGTCGT7750.032.94581651
CTCGATA7650.032.91229265
GCTCGTC7850.032.51313439
CGACGAG4850.030.57901224
GCTCGAT8250.030.51928364
TCGTCTA8400.029.97788854
GTCGTCT8650.029.90487153
TCGATAA8650.029.10740766
CTGGTCG9150.028.28202250
CGTCATT9250.027.96509226
GTCAGCG8900.027.90224843
CATTGCG9600.027.30480629
ACGATCG2200.026.6558460
TCAGCGC9850.026.61179244
AGGTCGT9850.026.26163722
CAATGCG5650.026.2492419
CGCGTAC951.0537129E-525.4138931
CGTCTAC10050.025.3993855
TAGGTCG10100.024.9286221
Produced by FastQC (version 0.11.5)