FastQCFastQC Report
Thu 26 May 2016
ERR1356995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743452
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG6650.041.5017924
CAATGCG6650.041.4994119
ATCGTCA7400.036.36105310
TGGTCGT9650.035.4012551
GCTCGAT9950.034.32303664
TCGTCTA10100.034.1675854
TGCGACG8100.034.0724622
GTCGTCT10350.033.34227853
CTGGTCG10350.033.34036350
GTCAGCG10550.033.02402543
GTCTACC10400.032.8501656
AGTCCTA8700.031.71899429
CTCGATA10850.031.47596465
GTACTAG9850.031.197971
GCGCTGG11100.031.07960747
CGATAAT10900.030.38300967
TCGATAA10950.030.24427466
CCAATGC9400.030.09172818
ATGCGAC9900.029.96827521
GCTCGTC11750.029.94407839
Produced by FastQC (version 0.11.5)