FastQCFastQC Report
Thu 26 May 2016
ERR1356995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743452
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG7550.036.54202324
ATCGTCA7650.035.68826310
CAATGCG8000.034.91761819
GTACTAG10000.030.7468051
GTCAGCG9250.029.45336543
TAGGCAT12700.028.791475
TGGTCGT9500.028.69555951
TGCGACG9700.028.44250322
GTCCTAC9750.027.94293630
GCTCGAT9550.027.81068464
CTGGTCG9850.027.32559250
CGTCATC10850.027.07378212
GTCTACC10050.026.77872556
TCGTCTA10050.026.77641954
GCGCTGG10350.026.67232147
GTCGTCT10150.026.50196853
CTAGGCA13800.026.4964964
ACCGTCG6200.026.14301723
CAGACCG6200.026.14301720
AGTCCTA10650.025.90537629
Produced by FastQC (version 0.11.5)