FastQCFastQC Report
Thu 26 May 2016
ERR1356994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967102
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG3850.035.84097324
GCTCGAT5750.034.7969164
TGGTCGT5950.033.63496451
CTCGATA6000.033.34788565
CGATAAT6050.033.07228567
TCGTCTA6350.032.0628754
GTCGTCT6250.032.02374353
TCGATAA6250.032.0139766
CAATGCG4450.031.00848419
CATTGCG6900.030.49573929
ATCGTCA4900.028.86478410
CTGGTCG6950.028.79539950
CGTCATT6950.028.78807826
GCTCGTC6900.028.49674639
TATCGGC2250.027.619322
CGTCTAC7700.026.44145855
GTCAGCG7450.026.39497243
GTCTACC8050.026.1491856
TAGGTCG7950.026.03542321
GTCGTCA7950.026.03542324
Produced by FastQC (version 0.11.5)