FastQCFastQC Report
Thu 26 May 2016
ERR1356994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967102
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA5100.031.78733865
CGATAAT5100.031.78733867
CGACGAG3800.031.76630824
GCTCGAT5200.031.17683464
TGGTCGT5400.030.6741651
TCGATAA5550.029.83147266
CAATGCG4350.027.74987819
GCTCGTC5950.027.24351939
TCGTCTA6400.025.87079454
CTGGTCG6450.025.68069350
CGTCTAC6600.025.61012555
ATCGTCA4650.025.2647410
TCAGCGC6350.024.98425344
ATGCGAC5600.024.63509621
TGCGACG5100.024.3452722
GTCGTCT6850.024.16756853
CATTGCG7150.024.11827529
GTATCAA55800.023.9551981
GTCAGCG7100.023.31659743
CGTCATT7450.022.68412826
Produced by FastQC (version 0.11.5)