FastQCFastQC Report
Thu 26 May 2016
ERR1356991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321298
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG5600.039.42647624
CAATGCG6200.033.9402819
ATCGTCA6700.031.40742710
CGATAAT8600.030.48872667
GCTCGAT8200.030.2904264
TGGTCGT9050.028.97895651
TCGATAA8900.028.68572666
CTCGATA8800.028.61779865
ATGCGAC8600.028.07990621
TGCGACG7650.027.959322
CTGGTCG9600.027.67816250
CGTCTAC9800.027.11446855
GTACTAG8800.027.0724541
GTATCAA59700.025.6206761
TCGTCTA10250.025.25072554
GGTATCA38500.025.1106851
GTCGTCT10550.024.53322453
GACGAGT9150.024.50689725
GTCAGCG10500.024.31351943
GCTCGTC10450.024.09452639
Produced by FastQC (version 0.11.5)