FastQCFastQC Report
Thu 26 May 2016
ERR1356991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321298
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGT9600.033.7928851
TCGATAA9450.032.84792366
CGATAAT9850.032.5644667
GCTCGAT9700.032.00339564
CGACGAG5350.030.94191624
GTCGTCT10400.030.83964353
TCGTCTA10750.030.16612254
CTCGATA10350.029.99222665
CAATGCG5850.029.4763619
CTGGTCG11200.028.96532650
CGTCTAC11200.028.95596355
ATCGTCA5900.028.1101810
TCAGCGC12000.027.58987644
GTCTACC11750.027.01333256
CATTGCG12450.026.86965829
GTCAGCG11800.026.59617443
GCGCTGG12250.026.48258247
GCTCGTC12000.026.44029839
CTCGTAT5500.026.33578939
GGTATCA42900.026.181481
Produced by FastQC (version 0.11.5)