FastQCFastQC Report
Thu 26 May 2016
ERR1356988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093429
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4300.037.70777524
CAATGCG4700.033.0305819
TGCGACG5050.032.79075222
CGATAAT5900.031.57499167
CTCGATA6350.029.33739565
TGGTCGT6800.027.90928351
GCTCGTC6700.027.28537639
ATCGTCA6100.027.14579210
TCGTCTA6900.027.00471354
GCTCGAT7100.026.23837364
ATGCGAC7050.025.44577821
CATTGCG7500.025.29534729
TCGATAA7400.025.17465866
CTGGTCG7550.025.13683950
GTCGTCT7400.024.71436353
GTCAGCG7400.024.70905143
TCAGCGC7700.023.74635944
ACCGTCG4250.023.54022223
GTACTAG7750.023.1657141
CGTCTAC8200.022.72347655
Produced by FastQC (version 0.11.5)