FastQCFastQC Report
Thu 26 May 2016
ERR1356988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093429
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA6100.036.187765
GCTCGAT6150.035.333564
CGATAAT6250.034.76733467
CGACGAG3900.033.605424
TCGTCTA6600.032.92911554
GCTCGTC6800.031.95374139
CGTCTAC6900.031.49816755
TTTCGCG450.004630272330.6575636
GTCGTCT7250.030.44612953
TGCGACG4250.030.02637322
TGGTCGT7250.029.98831451
GTCAGCG7400.029.82897643
CGTCATT7400.029.82897626
CTGGTCG7600.029.06135750
TCGATAA7500.028.97277566
CAATGCG4400.027.43503219
CATTGCG8000.027.1606829
AGGTCGT7900.027.0679122
TCAGCGC8150.026.66079144
ACCGTCG4800.026.58585223
Produced by FastQC (version 0.11.5)