FastQCFastQC Report
Thu 26 May 2016
ERR1356985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247360
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT3600.039.28842567
GCTCGAT3650.037.80358564
TGGTCGT3750.036.81172651
CTCGATA3750.036.7954965
CTGGTCG3850.036.75196550
TCGATAA3800.036.3127966
CAATGCG2950.035.08185219
CGACGAG2900.034.49853524
TCGTCTA4050.034.08629654
GCTCGTC4150.034.07605739
AGGTCGT4400.032.93042422
GTCGTCA4650.031.9018724
CGTCATT4450.031.78389226
GTCAGCG4800.031.6223943
GTCGTCT4500.031.44460953
CATTGCG4650.030.41562529
ATCGTCA3450.028.99644310
CGTCTAC4450.028.69568355
GTACTAG4950.027.8999821
CGCCAGC5100.027.72855834
Produced by FastQC (version 0.11.5)