FastQCFastQC Report
Thu 26 May 2016
ERR1356985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247360
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT2950.035.078267
CTCGATA3050.033.92809765
GCTCGAT3100.033.3808764
CGACGAG3200.032.3338324
TCGATAA3350.030.8897666
TGGTCGT3450.030.00402851
TCGTCTA3550.029.15066154
ATCGTCA3550.028.22763610
CGCTTCG500.007746755627.59153422
ACCGTCG2950.026.89005523
CTGGTCG3850.026.88672450
GGTATCA19900.026.5745121
CAATGCG3650.026.45763619
TGCGACG3750.025.75209822
CGTACGA3100.025.59302760
GTCAGCG4350.025.37152543
GTCGTCT4100.025.2361653
GGCGTCG3150.025.2302848
TCAGCGC4400.024.29936244
GCTCGTC4300.024.06238639
Produced by FastQC (version 0.11.5)