FastQCFastQC Report
Thu 26 May 2016
ERR1356981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130396
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT5850.038.32856464
CGACGAG5200.036.48907524
CAATGCG5150.036.17185619
TGGTCGT6300.035.05411551
ATCGTCA5550.034.80956310
GTCAGCG6350.033.6838343
TCAGCGC6700.031.92422744
GCGCTGG7150.031.36528647
CTCGATA7200.031.14333565
TCGTCTA7200.030.6737154
ACGGTCC450.004623470430.66556713
GTACTAG6900.030.5110531
GCTCGTC7350.030.50640939
CTGGTCG7300.030.25218450
TCGATAA7300.030.24415266
GTCTACC7650.030.22262656
TGCGACG6350.029.88081722
GTGTACG854.4155895E-628.4220621
GTCGTCT7800.028.31419453
CGATAAT7750.028.04291267
Produced by FastQC (version 0.11.5)