FastQCFastQC Report
Thu 26 May 2016
ERR1356981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130396
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA150.002353326469.01633547
CAATGCG5150.040.85386319
CGACGAG5200.039.7977424
ATCGTCA5250.039.46759810
CGATATA709.741616E-734.4974862
CGCGAAA400.00259893934.492918
GCTCGAT6500.033.4330664
GTACTAG7000.033.0790181
TGCGACG6550.031.59515422
TGGTCGT7000.031.55032351
GTCAGCG7150.031.35579543
CGTAGAC1001.45100785E-831.0477313
GTCATCC6700.030.8905313
GCTCGTC7900.029.68877839
GAGTCCT7100.029.63343228
GTCGTCT7450.029.63017553
GTCTACC7500.029.43915256
TAGGCAT9750.029.3670855
TCAGCGC7650.029.30639644
CTCGATA7550.029.2377265
Produced by FastQC (version 0.11.5)