FastQCFastQC Report
Thu 26 May 2016
ERR1356980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719056
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT3150.035.04463664
CGACGAG3550.033.04328524
CTCGATA3350.031.92358465
CAATGCG3500.030.55542819
TGCGACG3700.029.8387722
GTCGTCT3850.029.57751553
ATCGTCA3950.027.95023710
TCGATAA3900.027.42153566
ACCGTCG3450.027.00084123
TGGTCGT4800.026.59922451
CGCTCGA658.6572685E-426.53929110
ATGCGAC4250.025.97728221
CCGTCGG3600.025.87580724
CGTACGA3400.025.3647560
GCTCGTC4750.024.69119639
CATTGCG4700.024.21993329
GTCAGCG4850.024.18350843
GACCGTC3900.023.88535922
TCAGCGC5450.023.42002144
CTAACGC3100.023.3812223
Produced by FastQC (version 0.11.5)