FastQCFastQC Report
Thu 26 May 2016
ERR1356980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719056
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA2900.030.92576665
CGACGAG2550.029.7553124
GCTCGAT3250.027.59529964
TAGGCCG809.5737196E-525.8713465
TGCGACG2950.025.7206922
GTCGTCT3850.024.18716653
CAATGCG3000.024.14237619
TAACGCC2600.023.8812434
CTAACGC2750.023.8329963
TCTAACG2750.023.8329962
ACCGTCG2750.023.82883823
CGTCATT4350.022.99273926
CATTGCG4300.022.45802529
ATGCGAC3250.022.2852721
TAGTCGG1554.1030944E-822.25103840
GACCGTC3600.021.07667522
GCTCGTC4600.020.9933739
CGACTCG1657.936433E-820.90552965
TCGATAA4300.020.85691366
ATTCGTC2501.8189894E-1220.69346631
Produced by FastQC (version 0.11.5)