FastQCFastQC Report
Thu 26 May 2016
ERR1356979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770048
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG3100.034.4979224
CTCGATA3650.034.0253565
CAATGCG3800.029.05006419
GTACTAG5150.028.8220081
CGATAAT4450.028.68366667
GCTCGAT4400.028.22557464
GCTCGTC4800.028.0287739
TGGTCGT4700.027.89745751
TCGTCTA4550.027.30123154
TCGATAA4700.027.15793866
CGACTAT3050.027.14514236
CATTGCG5250.025.6255829
GTCGTCT5000.025.53423353
CTGGTCG5350.024.50804550
ATGCGAC4800.024.4360321
CGTCATT5600.024.02533726
TGCGACG4500.023.76523422
AGGTCGT5550.023.62020122
TAGGTCG5650.023.20214321
GTCAGCG5850.022.99861543
Produced by FastQC (version 0.11.5)