FastQCFastQC Report
Thu 26 May 2016
ERR1356979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770048
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG3600.039.28040724
CAATGCG4450.031.77740919
TGCGACG4750.029.77041422
TATGCGA854.3883465E-628.4462112
CTCGATA4750.027.5944365
GTACTAG6200.027.3154141
ATGCGAC5700.027.22903421
TCGATAA4650.026.70428766
ATCGTCA5400.026.22546210
CGATAAT4900.025.34182467
GCTCGAT5050.025.27212564
CGCACTA5100.025.02860358
ACCGTAT1251.2509372E-724.8694118
ACGAGTC6100.024.31270226
ACCGTCG3550.024.28881323
AATGCGA6200.023.92056320
GTCCTAC5850.023.58298330
TAGGCAT7900.023.5808495
GCTCGTC5600.022.7881139
GCGCACT5850.022.40953357
Produced by FastQC (version 0.11.5)