FastQCFastQC Report
Thu 26 May 2016
ERR1356977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270105
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT5000.026.21936267
CTCGATA5150.026.12558265
GCTCGAT5150.026.1244364
GGTATCA35450.025.0321881
CGACGAG3450.024.99939224
CGCGATA2150.024.06706214
TCGATAA5750.023.39943166
GTATCAA53550.023.2771431
TCGTCTA5600.022.8001254
CATTGCG5950.022.60841229
CAATGCG4300.022.46358119
CGTACGA4150.022.44474260
CTGGTCG5850.022.4131750
ACCGTCG4350.022.20635823
GTCGACG2650.022.13495350
CGTCTAC5950.022.03890655
GCTCGTC5850.021.81565739
CGTCATT6050.021.6665126
TGCGACG4300.021.66226622
GTCGTCT5700.021.189353
Produced by FastQC (version 0.11.5)