FastQCFastQC Report
Thu 26 May 2016
ERR1356977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270105
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG3150.028.46654724
GCTCGAT4000.027.59308464
CGATATA658.6645875E-426.53590262
CGTACGA3000.026.44919860
TGCGACG3550.026.2305522
GGTATCA33600.026.2221051
ACCGTCG3150.025.18194623
GTATCAA52150.024.9115161
CTCGATA4450.024.80222565
CGATAAT4450.024.80113267
ATCGTCA3800.022.7322410
CAATGCG4150.022.4381819
TCGATAA4800.022.27468766
TCGTCTA5200.021.89115354
CAACGCA57300.021.6126235
ATCAACG57650.021.6010843
TATCAAC60400.021.1887152
TCAACGC59650.020.9346524
GCTCGTC5300.020.82313339
AACGCAG60800.020.6295096
Produced by FastQC (version 0.11.5)