FastQCFastQC Report
Thu 26 May 2016
ERR1356975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702605
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG5700.032.68276224
CAATGCG5900.032.1586519
GCTCGAT7550.031.984464
ATCGTCA5800.031.5235410
TGGTCGT8200.030.72017751
GAACCGT953.2074058E-729.0504916
GTCAGCG9150.028.65362543
TGAACCG1503.45608E-1127.6093225
CTCGATA9000.027.21466365
GCTCGTC9650.026.80834639
CGATAAT8950.026.59580667
GTCTACC9450.026.29150456
CTGGTCG9750.026.1903850
GTCGTCT9800.026.05675753
GCGCTGG10100.025.95924647
TCAGCGC10550.025.1782444
CGCCAGC10750.025.02777334
TCGATAA10100.024.2506966
TGGACGA2001.8189894E-1224.1482210
GCACCGG1452.0179868E-823.7913516
Produced by FastQC (version 0.11.5)