FastQCFastQC Report
Thu 26 May 2016
ERR1356975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702605
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG5300.039.04458619
CGACGAG5400.037.0441524
TGGTCGT6900.034.5035751
ATCGTCA5850.034.2549610
CGATCGA851.2094642E-732.46917754
GCTCGAT7200.031.14082164
TCAGCGC7800.030.95201144
GCTCGTC7650.030.6572339
CTGGTCG8050.030.43172350
CGACACT1600.030.17998514
TGGACGA1650.029.31537410
GTCAGCG8150.029.19960243
GTCTACC7800.028.74959456
ATCGCCG605.4330577E-428.74115439
GTCGTCT8250.028.42761253
GCGCTGG8550.028.24853347
CGCCAGC8450.028.16292834
ATCGCCA500.007747571427.59150727
CTCGATA8300.027.0129365
CGGTCCG1155.6594217E-826.9916928
Produced by FastQC (version 0.11.5)