FastQCFastQC Report
Thu 26 May 2016
ERR1356973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636513
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4350.037.27310624
CAATGCG4700.034.49744819
TGCGACG4950.032.7551522
CGATAAT5550.032.3229267
TGCGTTA901.9952131E-730.66814651
ATCGTCA5550.030.45813810
TGGTCGT6250.030.36146551
GTACTAG6950.028.8103661
GTCTACC6500.028.66379756
TCGTCTA6750.028.62448154
TCGATAA6400.028.56907366
CGATCAA854.4364224E-628.4061933
CTCGATA6350.028.25074265
GCTCGTC6500.028.12868939
GCTCGAT6400.028.03003364
CGTCATT6800.027.90149526
CTGGTCG7200.026.83462750
GTCAGCG6700.026.77577643
GTCGTCT7100.026.7274653
ATGCGAC6200.026.707721
Produced by FastQC (version 0.11.5)