FastQCFastQC Report
Thu 26 May 2016
ERR1356973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636513
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT306.3205947E-445.9876624
CGACGAG4750.037.75828624
CGTCATT7200.033.05362726
CAATGCG5500.032.60943219
GCTCGTC7650.032.01101739
GTCGTCT7250.031.39846853
CTCGATA6950.031.26690165
TGCGACG5750.031.1916322
TCGTCTA7650.030.21127354
TCGATAA7250.029.97309966
GTCTACC7600.029.9561556
ATCGTCA5900.029.85873410
CGATAAT7050.029.8448867
GTACTAG6850.029.7755851
GTCAGCG8350.029.32745743
GCTCGAT7500.028.97576564
CATTGCG8700.028.94050629
AGGTCGT8450.028.57221222
TGGTCGT8000.028.46703751
CGTCTAC8100.028.10700255
Produced by FastQC (version 0.11.5)