FastQCFastQC Report
Thu 26 May 2016
ERR1356972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819698
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG3200.029.10776322
CGACGAG3450.027.99845124
CAATGCG3600.025.87214519
ACCGTCG2950.025.72738823
TCGTCTA4250.024.35694954
TGGTCGT4400.024.31081651
ATCGTCA4050.023.84987410
CGATAAT4250.023.53987367
ATGCGAC4900.023.23340821
CCGTCGG3350.022.65546224
TCGATAA4300.022.46387166
GTACTAG5150.022.126861
CTCGATA4400.021.95272665
CGTACGA3500.021.68391860
ATACGGT800.00290146921.5607136
CATTGCG4650.021.51316329
GTCCTAC6250.020.4425351
TAGCGTA3900.020.34782457
TAATACG850.004128320620.28797533
GTATCAA44200.020.078161
Produced by FastQC (version 0.11.5)