FastQCFastQC Report
Thu 26 May 2016
ERR1356972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819698
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG3050.037.3166322
CGACGAG3400.034.48961324
CAATGCG3850.030.45835919
TCGTCTA5150.027.46378954
CTCGATA5050.027.32225865
CGATAAT5150.026.79099567
ATCGTCA4700.025.72900210
GCTCGAT5400.025.55207464
TCGATAA5700.024.20589866
TGGTCGT5750.024.00200751
GTCGTCT5850.023.58264253
CGTCTAC6150.022.99876255
ACCGTCG3750.022.99307623
TCGTATG3200.022.63380840
CTGGTCG6300.021.90659350
ATGCGAC6800.021.80960821
TATGCCG3350.021.62035643
CATTGCG6550.021.58891929
CGTCATT6900.021.49352826
GCTCGTC6800.021.30240839
Produced by FastQC (version 0.11.5)