FastQCFastQC Report
Thu 26 May 2016
ERR1356969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384169
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG5400.033.86184322
CTCGATA7000.033.512265
GCTCGAT7400.031.23453964
TGGTCGT7800.029.64085251
CGATAAT7800.029.6340167
CAATGCG6300.029.02382919
TCGATAA8350.027.68207266
TCGTCTA8450.027.36136254
ATCGTCA6750.027.0877710
GTACTAG8750.026.8243891
GCTCGTC8900.026.74159639
CTGGTCG9000.026.45556450
GTCGTCT8850.026.1246953
GTCAGCG9000.026.06668343
CGTCTAC9200.025.88098755
ATGCGAC7700.024.64338921
CGTCATT10050.024.3695726
GCATCGT7900.024.0195068
CATTGCG10150.023.78862629
AGGTCGT10300.023.78206622
Produced by FastQC (version 0.11.5)