FastQCFastQC Report
Thu 26 May 2016
ERR1356969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384169
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG6000.034.49102424
CTCGATA7050.033.27072565
CAATGCG6800.031.95492219
CGATAAT7400.031.69711167
TGGTCGT7850.031.65167451
TGCGACG6600.031.35547622
CGGCGAA450.004629566330.65868841
ATCGTCA7050.030.37138610
GCTCGAT7950.029.50423264
GCTCGTC8800.029.39575839
GTCAGCG8900.028.67792943
CGTCATT9050.028.20260426
CATTGCG9250.027.96569629
CTGGTCG9150.027.53186450
GTCGTCT9150.027.51742753
TCGTCTA9250.027.22679754
TCGATAA8750.027.20091866
AGGTCGT9600.026.58683222
ACGAGTC8200.026.49920326
TCAGCGC9850.025.91203944
Produced by FastQC (version 0.11.5)