FastQCFastQC Report
Thu 26 May 2016
ERR1356967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991463
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA6900.031.50344365
GCTCGAT7450.029.63560964
TCGATAA7450.029.17768366
TCGTCTA7900.028.3934654
TGGTCGT7850.028.13470351
CGATAAT7950.026.04058367
CTGGTCG8750.026.02766450
GTCGTCT8750.025.2408553
CAATGCG6700.024.19987919
TGCGACG6800.023.84340122
GTCAGCG9500.023.60250543
ATCGTCA6900.023.50020410
GTACTAG7550.023.3224261
GCTCGTC9600.022.9874939
GTCTACC9850.022.7724256
CGTCATT9900.022.29818226
TAGGCAT11200.022.1932685
CATTGCG10500.021.35625629
CGTCTAC10450.021.13468455
GCGCTGG10900.020.88694247
Produced by FastQC (version 0.11.5)