FastQCFastQC Report
Thu 26 May 2016
ERR1356967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991463
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGT7200.030.1947151
CTCGATA7300.028.83782865
TGCGACG6050.028.49987422
GCTCGAT7550.027.88293364
CGACGAG6100.027.70094324
TCGATAA7750.027.16337466
GTCGTCT8300.026.59072753
TCGTCTA8450.026.13904654
CTGGTCG8500.025.57669450
CGATAAT8200.025.24485867
CGTCATT9200.024.7391326
CAATGCG6700.024.70556319
ATCGTCA7300.022.70575110
GCTCGTC9800.022.52071839
CGTGGTT6350.022.26580636
GTCTACC10050.021.9809256
TCAGCGC10250.021.86843944
CATTGCG10100.021.85178629
CGCCAGC10200.021.63755234
GCGCTGG10300.021.44201547
Produced by FastQC (version 0.11.5)