FastQCFastQC Report
Thu 26 May 2016
ERR1356964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541693
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG7150.037.63347619
ATCGTCA7300.035.91505410
GTATTGA601.3337241E-534.5343441
CGACGAG7900.033.62400424
TGGTCGT9100.030.33916351
TGCGACG9000.029.89770922
GGTTCGG605.429603E-428.74406442
GAGTCCT9500.028.326928
GTCATCC9650.027.88206313
GTCAGCG9700.027.7356443
ACGTCCC500.00773861827.59788327
GTCTACC10050.027.4712856
GTCGTCT10100.027.33528353
CCAATGC10100.027.32463818
GCTCGAT9500.027.23740664
CTCGATA9950.026.35658365
GTCCTAC10150.026.17125530
TCATCCA10600.025.7094614
TCGATAA10600.025.06590866
TGTTTCG1801.2732926E-1124.92122866
Produced by FastQC (version 0.11.5)