FastQCFastQC Report
Thu 26 May 2016
ERR1356964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541693
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG200.007346817351.7725959
CGACGAG7650.040.57434524
CAATGCG7700.039.8642719
ATCGTCA8000.039.27873210
CGCTGCG709.705909E-734.5150657
TGGTCGT9400.034.50386451
TGTACGA400.002600556534.4893118
CGGCGAT807.118433E-834.48819732
GAGTCCT9350.033.56604828
TGCGACG9650.032.16515722
GTCATCC9900.031.70332513
GTACTAG11200.031.4691091
GTCCTAC10050.031.22811730
ATGTACG450.00463018730.6571717
GTCAGCG10550.030.40191543
GCTCGTC10900.030.0585239
GCGCTGG10800.030.0311447
GCTCGAT10750.029.84791464
CTCGATA10950.029.61783265
GTCGTCT11300.029.29970653
Produced by FastQC (version 0.11.5)