FastQCFastQC Report
Thu 26 May 2016
ERR1356961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590223
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT9550.034.6866267
GCTCGAT9700.034.1436364
CTCGATA9800.033.44965765
TCGATAA9950.033.29218366
TGGTCGT10450.032.36153451
TCGTCTA10900.030.7089254
GTCGTCT11700.028.90410453
CGTCTAC11900.028.4183255
CTGGTCG12050.027.77550950
GTCAGCG12450.027.15502443
TCAGCGC12400.026.98630744
GTCTACC12750.026.79441656
GCTCGTC13150.025.17558539
TGCGACG7750.024.92563422
CGTCATT14200.023.5627826
GTACTAG10200.023.3549021
CGACGAG8350.023.13546624
GCGCTGG15350.022.47293947
AGGTCGT15050.022.46205322
CATTGCG14750.022.4529129
Produced by FastQC (version 0.11.5)