FastQCFastQC Report
Thu 26 May 2016
ERR1356961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590223
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG7850.029.43535624
CTCGATA8950.028.91369865
ATCGTCA8300.028.70484710
CAATGCG8050.028.27617319
CGATAAT9000.027.98415667
TGCGACG8200.027.75838922
TCGATAA9600.026.59658666
GCTCGAT9850.026.27234664
TCGTCTA10850.025.76049654
TGGTCGT10650.025.27127551
GCTCGTC12250.023.64870639
CGTCTAC11400.023.60960255
TAGGCAT13000.023.3500215
ATGCGAC10850.023.20370121
GTACTAG11200.023.1408961
GTCGTCT11850.022.99179653
GTCTACC12100.022.81763856
CGTCATT13550.022.1433926
AGTCCTA10800.022.03380429
CTGGTCG12300.021.88122750
Produced by FastQC (version 0.11.5)