FastQCFastQC Report
Thu 26 May 2016
ERR1356960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892529
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG5850.029.48180224
ATCGTCA6000.028.1728410
CAATGCG6300.026.83056619
GCTCGTC9800.025.68614239
GCTCGAT8900.025.58298564
GTCAGCG10000.025.52669343
CTCGATA9050.025.16694365
TGGTCGT9350.024.72983751
TCGATAA9100.024.6494466
GTCTACC10150.024.4807456
CGATAAT9350.023.99036467
TGCGACG7200.023.95396422
TCGTCTA9850.023.82488354
GTCGTCT9850.023.82488353
CGTCATT11150.023.2008226
GTACTAG8950.022.7593271
TCAGCGC11700.021.81768644
CTGGTCG10800.021.728650
ACGTTAA2700.021.7199848
AGTCCTA8100.021.29522929
Produced by FastQC (version 0.11.5)