FastQCFastQC Report
Thu 26 May 2016
ERR1356960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892529
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG6750.032.6995424
ATCGTCA7950.028.22889310
CAATGCG8000.028.02133419
GCTCGTC10700.027.39684739
TGGTCGT10300.027.13652451
TGCGACG8350.026.84678622
CGTCATT10950.026.45638826
GTCTACC11300.025.96066556
CTCGATA11050.025.60695865
TCGATAA11100.025.49228766
GTCAGCG11250.025.44432843
GCTCGAT11200.025.2640164
CTGGTCG11150.025.06782250
GTCGTCT11250.024.83121153
GTCCTAC9450.024.45166430
GTACTAG10050.024.0569421
TCGTCTA11950.023.96716354
CGATAAT12000.023.86540867
TCAGCGC11900.023.764744
CATTGCG12350.023.45728529
Produced by FastQC (version 0.11.5)