FastQCFastQC Report
Thu 26 May 2016
ERR1356957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652980
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG11200.034.18994519
ATCGTCA11550.032.85396610
TGGTCGT14150.032.67992851
CGACGAG12300.032.2517624
GTCAGCG15550.031.27993243
CTCGATA15400.030.24970865
GTCGTCT16100.028.93614253
TCGATAA16100.028.72017366
GCTCGAT16350.028.27409464
TGCGACG14100.027.644722
GTCTACC17000.027.40422856
GCGCTGG17750.027.40246847
TCAGCGC18300.026.76790244
TCGTCTA17900.025.83357454
GCTCGTC18850.025.24675639
GAGTCCT15400.024.6409428
GTCCTAC15500.024.4810430
GTACTAG17000.024.3717271
CGCCAGC20250.024.01672734
CTGGTCG20550.023.6759450
Produced by FastQC (version 0.11.5)