FastQCFastQC Report
Thu 26 May 2016
ERR1356957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652980
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA10700.037.75757210
CGACGAG11550.034.63742424
TACCCCG601.3455097E-534.491385
CAATGCG11450.034.33752419
TGGTCGT16700.033.2665951
GTCGTCT17500.031.92615153
CGCGAAA450.00463156230.6561118
GTCAGCG18800.030.45228243
CTCGATA18300.029.97808865
GCTCGAT18550.029.7600764
GTCTACC19200.029.65779956
TATTCGC1202.6630005E-928.7753589
TGCGACG13800.028.49019222
GCGCTGG19900.028.43738247
TCGATAA19600.028.16578366
TCAGCGC20750.027.42429444
GCTCGTC20800.027.3583739
TCGTCTA20500.027.26842154
GTCCTAC14850.026.47573530
CGTCATT22000.026.0228626
Produced by FastQC (version 0.11.5)