FastQCFastQC Report
Thu 26 May 2016
ERR1356955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751599
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA7550.031.52827310
CGACGAG7550.031.06956924
CAATGCG7450.031.02268419
TGGTCGT8800.029.41543251
GCTCGAT9350.028.04302464
GTCTACC9500.027.9745956
TCGTCTA9500.027.61128454
CTGGTCG9650.027.1782150
CTCGATA9900.026.49037765
TGCGACG9200.025.8715322
AGTCCTA9500.025.42127229
GTACTAG10700.025.1698861
GCTCGTC11000.024.76687439
CGTGGTT9250.024.60588636
GTCCTAC9450.024.45983130
TCGATAA10800.024.28284566
GACCGTC9450.024.09200722
ACCGTCG9500.023.9658923
CGTCATT11750.023.77775226
GTCGTCT11150.023.5253153
Produced by FastQC (version 0.11.5)