FastQCFastQC Report
Thu 26 May 2016
ERR1356955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751599
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG1003.8198777E-1034.4983865
CGACGAG7650.034.26312324
CAATGCG7750.033.3769619
TGGTCGT9550.031.7981751
ATCGTCA7950.031.70401610
CTGGTCG10500.029.90714350
GCTCGTC11050.029.02655439
GCTCGAT10800.028.10578364
CGTCATT11550.028.06859426
GTCCTAC9250.027.9636830
TCGTCTA11250.027.29439454
GTCTACC11300.027.18060756
AGTCCTA9700.027.0219529
CTCGATA11550.026.28073365
GTCGTCA12250.026.18313624
GTCAGCG12550.026.10686143
TGCGACG10050.026.08088522
ATTAGGT12800.025.59769219
CGTGGTT8650.025.5175336
CGGCGAT951.0538901E-525.41260732
Produced by FastQC (version 0.11.5)