FastQCFastQC Report
Thu 26 May 2016
ERR1356954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604851
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG53550.33367583657299027No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC30790.19185581714439534No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG23680.14755263884310754No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA21960.13683513298119265No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA21040.13110251356668No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA20850.12991860303542196No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT19180.11951265257646972No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG17740.1105398569711456No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG17440.10867052455336974No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG16880.10518110404018817No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGG200.00735263451.76232566
GTACTTG350.001344336939.4502681
CAATGCG9350.036.52672219
ATCGTCA9300.035.9823410
TGGTCGT11550.035.56057751
GAGTCCT9550.035.0425828
TACCCCG1102.7284841E-1134.517915
CGGTGTT905.1950337E-934.49853514
GCCGAAT400.002597975334.4963862
CCGAATT400.002597975334.4963863
CACGTTA400.002600359334.4899360
GCTCGTC12200.034.48348239
CGACGAG9750.033.9667324
GCAGCGA1253.6379788E-1233.11859515
GCTCGAT12000.033.06212664
GTCAGCG12800.032.87731643
GTCTACC12850.031.96300956
TAAGGGT553.247105E-431.3808944
GCGACGT1550.031.14831235
CCGAGTA450.004624897630.66440848
Produced by FastQC (version 0.11.5)