FastQCFastQC Report
Thu 26 May 2016
ERR1356954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604851
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG55020.34283556542009197No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA36590.2279962438880619RNA PCR Primer, Index 46 (96% over 28bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC30180.18805484122825109No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG23600.14705415019836732No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA22140.13795673243185816No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA21120.13160100221142024No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA20850.12991860303542196No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT17960.11191070074418125No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG17300.10779816942507435No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG16430.10237710541352438No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG16370.10200323892996921No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC306.307513E-446.00694354
GGCGAGC404.9984606E-543.13150851
CAATGCG7700.040.7598219
TCAAGAC1800.040.243533
CGACGAG8050.039.8445324
ATCGTCA8250.038.917410
GAGTCCT8200.038.69506528
GCGCGAG557.360173E-637.66317411
GCAGCGA952.2009772E-1036.30542415
TGGTCGT13500.035.78317651
ACCCTTG4450.034.9114047
GGCGCCA400.002587587834.5245812
CCAAATG400.002594719234.50520748
ACGACAG400.002594719234.50520745
CGCAGCG1003.8198777E-1034.49015814
ATCGCCG1003.8198777E-1034.4890839
GTCAGCG14750.033.67069643
CTACACT9050.033.53634333
GCTCGAT13950.033.3910964
GCTCGTC15400.032.24952739